The seven regional archives are the repositories for most pre-1900 parish books and, therefore, are the archives of primary importance to Czech genealogical researchers. In addition to early parish books, the regional archives house an enormous amount of important material including early cadastral records, maps, architectural plans, historical documents, court records, administrative papers, etc. The archives maintain extensive catalogs of their holdings, which are slowly being made available online.

The Central Bohemian Region is served by the State Regional Archive in Prague
Comprises the following districts: Benešov, Beroun, Kladno, Kolín, Kutná Hora, Mělník, Mladá Boleslav, Nymburk, Prague-country, Příbram, and Rakovník
Open Monday & Wednesday 9:00am to 6:00pm, Tuesday & Thursday 9:00am to 4:00pm.
Online digital records can be found at: http://ebadatelna.soapraha.cz/
District subsidiary archives

Prague
State Regional Archive in Prague
Archivni 4/2257
149 00 Prague 4 - Chodovec

The Southern Bohemian Region is served by the State Regional Archive in Třeboň
Comprises the following districts: České Budějovice, Český Krumlov, Jindřichův Hradec, Pelhřimov, Písek, Prachatice, Strakonice, and Tábor
Open Monday & Wednesday, 8:00am to 5:00pm; Tuesday & Thursday, 8:00am to 3:00pm.
Seats in the study room must be reserved at least one week in advance.
Online digital records can be found at: http://digi.ceskearchivy.cz/DA?lang=en
District subsidiary archives

Třeboň
State Regional Archive in Třeboň
Třeboň Zámek
379 11 Treboň

The Western Bohemian Region is served by the State Regional Archive in Plzeň
Comprises the following districts: Domažlice, Cheb, Karlovy Vary, Klatovy, Plzeň-north, Plzeň-south, Rokycany, Sokolov, and Tachov
Open Monday & Wednesday, 8:00am to 5:00pm; Tuesday & Thursday, 8:00 to 4:00pm.
Online digital records can be found at: http://www.portafontium.cz/
District subsidiary archives

Plzeň
State Regional Archive in Plzeň
Sedláčkova 44, p.p.3
12306 12 Plzeň

The Northern Bohemian Region is served by the State Regional Archive in Litoměřice
Comprises the following districts: Česká Lípa, Děčín, Chomutov, Jablonec nad Nisou, Liberec, Litoměřice, Louny, Most, and Teplice
Open Monday & Wednesday, 8:00am to 5:00pm; Tuesday, 8:00am to 3:00pm.
Online digital records can be found at: http://vademecum.soalitomerice.cz/vademecum/
District subsidiary archives

Litoměřice
State Regional Archive in Litoměřice
Krajská 1
412 74 Litoměřice

The Eastern Bohemian Region is served by the State Regional Archive in Hradec Králové
Comprises the following districts: Hradec Králové, Chrudim, Jičín, Náchod, Pardubice, Rychnov nad Kněžnou, Semily, Svitavy, Trutnov, and Ústí nad Orlicí
Open Monday & Wednesday, 8:00am to 5:00pm; Tuesday & Thursday, 8:00am to 3:00pm.
A list of parishes that have already been digitized can be found at: https://aron.vychodoceskearchivy.cz/
Copy the link for a specific parish book to your browser in order to download the entire book.
District subsidiary archives

HRADEC KRÁLOVÉ
State Regional Archive in Hradec Králové
Zámek
565 43 Zámrsk
&
Balbínova 821/8
500 03 Hradec Králové

The Southern Moravian Region is served by the Moravian Land Archive in Brno
Comprises the following districts: Blansko, Brno-venkov, Břeclav, Havlíčkův Brod, Hodonín, Jihlava, Kroměříz, Prostějov, Třebíč, Uherské Hradiště, Vyškov, Zlín, Znojmo, and Žďár nad Sázavou
Open Monday & Wednesday - 9:00am to 6:00pm; Tuesday & Thursday - 9:00am to 4:00pm. Closed during the month of July.
Online digital records can be found at: http://actapublica.eu/
District subsidiary archives

Brno
Moravian Land Archive in Brno
Palachovo náměstí 1
PO Box 51
625 00 Brno

The Northern Moravian Region is served by the Land Archive in Opava
Comprises the following districts: Bruntál, Frýdek-Místek, Jeseník, Karviná, Nový Jičín, Olomouc, Opava, Přerov, Šumperk, and Vsetín
Open Monday & Wednesday, 8:00am to 5:00pm; Tuesday & Thursday, 8:00am to 3:00pm. It's recommended to reserve a seat in the study room well in advance.
Online digital records can be be found at: http://vademecum.archives.cz/vademecum
District subsidiary archives

Opava
Land Archive in Opava
Sněmovní 1
746 22 Opava

Olomouc Branch (has records for the Olomouc district and parts of the surrounding districts)
u Husova Sboru
10771 11 Olomouc